mouse anti eea1 e 8 Search Results


mouse anti eea1 e 8  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse anti eea1 e 8
Mouse Anti Eea1 E 8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti lamp1 1d4b antibodies  (Santa Cruz Biotechnology)
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C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
Rat Anti Lamp1 1d4b Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rat anti lamp1 1d4b antibodies - by Bioz Stars, 2026-07
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mouse igg  (Abcam)
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Abcam mouse igg
C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
Mouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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f ab anti mouse alexa fluor 488 jackson immuno 115 547 003 goat  (Jackson Immuno)
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C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
F Ab Anti Mouse Alexa Fluor 488 Jackson Immuno 115 547 003 Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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f ab anti mouse unconjugated jackson immuno 115 007 003 goat  (Jackson Immuno)
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Jackson Immuno f ab anti mouse unconjugated jackson immuno 115 007 003 goat
C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
F Ab Anti Mouse Unconjugated Jackson Immuno 115 007 003 Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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f ab anti rabbit unconjugated jackson immuno 111 007 003 goat  (Jackson Immuno)
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C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
F Ab Anti Rabbit Unconjugated Jackson Immuno 111 007 003 Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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f ab anti rabbit alexa fluor 488 jackson immuno 111 547 003 goat  (Jackson Immuno)
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Jackson Immuno f ab anti rabbit alexa fluor 488 jackson immuno 111 547 003 goat
C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or <t>LAMP1</t> ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.
F Ab Anti Rabbit Alexa Fluor 488 Jackson Immuno 111 547 003 Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or LAMP1 ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.

Journal: International Journal of Molecular Sciences

Article Title: C-Terminal Domain of Aquaporin-5 Is Required to Pass Its Protein Quality Control and Ensure Its Trafficking to Plasma Membrane

doi: 10.3390/ijms222413461

Figure Lengend Snippet: C-terminal domain mutant of AQP5 localizes to autophagosome or lysosome and is degraded via autophagy. HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, calnexin ( A ) or TGN38 ( B ), by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A were treated with brefeldin A (( C ), BFA, 5 µg/mL) or monensin (( D ), Mon, 10 µM) for 12 h, after which the cells were washed out and incubated for 3 or 6 h. The cellular localization of the mutants was assessed by immunofluorescence. HEK-293 cells transfected with hAQP5 wild-type or L262A with LC3-GFP were analyzed for the cellular localization of the mutants and LC3 by immunofluorescence ( E ). HEK-293 cells transfected with hAQP5 wild-type or L262A were analyzed for the cellular localization of the mutants, p62 ( F ), or LAMP1 ( G ), by immunofluorescence. HEK-293 cells transfected with hAQP5 L262A were treated with MG132 (10 µM), bafilomycin A1 (BafA1, 500 nM), chloroquine (CQ, 20 µM), or ammonium chloride (10 µM) for 12 h. The cellular localization of the mutants was analyzed by immunofluorescence ( H ). The level of whole-cell mutants was analyzed by Western blotting. Each data point represents mean ± SE ( n = 3), * p < 0.05 vs. control ( I , J ). HEK-293 cells transfected with hAQP5 wild-type and L262A were treated with cycloheximide (100 µg/mL) for 1, 3, 6, or 12 h. The level of whole-cell mutants was analyzed by Western blotting. Each data represents mean ± SE ( n = 4), * p < 0.05 vs. control ( K ). Typical data in triplicated experiments are shown.

Article Snippet: Mouse anti-calnexin (AF18), EEA1 (E-8), TGN38 (B-6), and rat anti-LAMP1 (1D4B) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Mutagenesis, Transfection, Immunofluorescence, Incubation, Western Blot, Control